Do there is a protocol or proposed antibodies to execute ICC staining on my entirely differentiated ALI cultures?
When the operate is finish, retrieve the Supply Cartridge in the instrument. The cell sample ought to be at the bottom or side of the gathering tube. Note: It is recommended to spin down the Delivery Cartridge inside a mini-centrifuge for any couple of seconds for total volume recovery.
Get hold of us to debate customized assay methods for a number of other cell kinds to fit your distinct wants.
It's important to quench the dissociation reagent applying FACS buffer or quit medium, by including at the least a similar or double the volume of the dissociation reagent.
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Notes: Use pipette tricks to Carefully scratch The underside of each and every perfectly 자가지방이식 to be able to elevate the monolayer off, then dissociate the cell clumps into one-cell suspension by Stem cell pipetting up and down five - 10 occasions.
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